• Overall ten genes five of which are coding genes

  2018-11-08

  

  Overall, ten genes, five of which are coding genes (FMOD, SULF1, COL12A1, STMN2 and MAEL), and five long-non-coding RNA (lncRNA) genes, were differentially expressed in hUC cells. lncRNAs are non-protein coding transcripts longer than 200 nucleotides. They have been implicated in the regulation of g

• In contrast to bona fide ESC lines the pre

  2018-11-08

  

  In order Asiatic acid to bona fide ESC lines, the pre-ESC lines expressed H19 and IGF2 mRNAs and lower levels of DNMT3B. This may reflect their limited ability to differentiate into the three germ layers, as a high H19 expression level has been associated with altered differentiation potential in p

• blasticidin A microsatellite PCR profile has

  2018-11-08

  

  A microsatellite PCR profile has been obtained for the cell line, and HLA Class I and II typing is available (Table 1). Blood group genotyping gave the blood group O1O1, expected to give rise to blood group O+ (Table 1). The cell line is free from mycoplasma contamination as determined by RT-qPCR.

• The following are the supplementary data related to this art

  2018-11-08

  

  The following are the supplementary data related to this article. Acknowledgments The authors wish to thank Dr. K. Inoue for the S100β/GFP-TG rats, Dr. A.F. Parlow and the NIDDK for the mdm2 inhibitor against pituitary hormones, Dr. S. Tanaka at Shizuoka University for the antibody against huma

• To test whether off target effect occurred in Abcc A

  2018-11-08

  

  To test whether off-target effect occurred in Abcc8-A4, a total of 8 potential off-target sites (POTs) for each sgRNA were predicted by the CRISPR design online tool. All POTs were PCR amplified and then sequenced. Sequencing results showed that no mutation was detected in these POTS (Fig. S1.A, B),

• i iPSC colonies displayed a typical

  2018-11-08

  

  i10984 iPSC colonies displayed a typical ES-like colony morphology (Fig. 1B). Immunocyto-chemical analysis revealed expression of transcription factors OCT4 and SOX2, and surface markers SSEA4 and TRA-1–81, characteristic of pluripotent stem ikk inhibitor (Fig. 1B). The iPS cells exhibited a normal

• br Funding This work was funded by NIHR GM

  2018-11-08

  

  Funding This work was funded by NIHR01GM085456 awarded to Tanja Dominko. Declaration of interest The following are the supplementary data related to this article. Acknowledgements Introduction The identification of culture conditions that promote the expansion of primitive hematopoieti

• Cancer cells use epigenetic mechanisms to

  2018-11-08

  

  Cancer Aprotinin use epigenetic mechanisms to control tumor growth, invasion, and resistance to therapy. Novel therapeutic approaches targeting epigenetic modifications are considered the new frontier in cancer therapy. In fact, pharmacological modulation of epigenetic alterations is expected to ov

• sirtuin inhibitors The following are the supplementary data

  2018-11-08

  

  The following are the supplementary data related to this article. Conflict of interest Introduction Mammalian eyes contain retinal stem cells (RSCs) that are capable of proliferating and differentiating into all of the cell types of the neural retina as well as the retinal pigmented epitheliu

• br Materials and Methods br Results br Discussion The

  2018-11-08

  

  Materials and Methods Results Discussion The importance of bone marrow microenvironment in leukemogenesis becomes more evident each day. Kim and colleagues described the ability of leukemic stem ap-1 transcription factor to reset the communication between hematopoietic stem cells and the b

• Alternative approaches to LV are

  2018-11-08

  

  Alternative approaches to LV are non-viral vectors such as RNA or DNA, which are considered safer due to their integration-free nature (Hardee et al., 2017). However, manufacturing RNA is expensive and difficult because RNA has a propensity to degrade (Yin et al., 2014). On the other hand, DNA plasm

• Local connectivity was assessed in animals pre injected

  2018-11-08

  

  Local connectivity was assessed in animals pre-injected with lentiviral vectors into the striatum prior to grafting (Figure 4A). In these animals, a widespread mCherry expression in host striatum was observed (Figures 4B and 4C), and a distinct mCherry− graft core could be easily delineated (Figures

• br Experimental Procedures br Author Contributions M A plann

  2018-11-08

  

  Experimental Procedures Author Contributions M.A. planned and performed the experiments and wrote the manuscript. T.N. helped in performing CTL redifferentiation experiments. S.Y. provided scientific discussion and technical support. T.Y. provided plasmids and helped in generating lentivirus v

• The fate of hESC derived pancreatic progenitor

  2018-11-08

  

  The fate of hESC-derived pancreatic progenitor cells differed substantially following transplantation in SCID-beige mice versus nude rats. As early as 19 weeks post-transplantation, rats had significantly lower fasting human C-peptide levels and enhanced meal- and glucose-responsive C-peptide secret

• Both NHEJ and HDR have been used in hPSCs

  2018-11-07

  

  Both NHEJ and HDR have been used in hPSCs for gene editing (Byrne et al., 2015; Chen et al., 2014b; Ding et al., 2013a; Ding et al., 2013b; Genga et al., 2015; Hockemeyer et al., 2011; Hou et al., 2013; Kleinstiver et al., 2016; Slaymaker et al., 2016). For targeted gene knockout in hPSCs, NHEJ usua

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