• Although in previous studies by Soldner et

  2018-11-08

  

  Although in previous studies by Soldner et al. (2011) and Ding et al. (2013), an initial FACS-based enrichment step of TALEN-expressing phospholipase c inhibitor prior to subsequent PCR-based screening of this enriched cell population had to be included, both groups provided initial evidence that th

• Introduction The human epidermis is renewed

  2018-11-08

  

  Introduction The human oxygenase is renewed monthly, and daily occurring wounds need timely repair. The processes involved in this regeneration and repair rely on epidermal stem cells, which generate colonies known as holoclones (Barrandon and Green, 1987; Pellegrini et al., 1999a; Rochat et al.,

• Comparative gene expression analysis of ex

  2018-11-08

  

  Comparative gene expression analysis of ex vivo expanded lung stromal clemastine fumarate manufacturer also showed that the activation of an epithelial-supportive phenotype was associated with the reactivation of genes related to lung development, epithelial morphogenesis and angiogenesis. This sugg

• Environmental oxygen levels affect tissue

  2018-11-08

  

  Environmental oxygen levels affect tissue vascularization and fracture healing. A previous study suggested that hyperoxia (50% O2 and 1ATA) increased tissue vascularization but did not significantly alter osteogenesis during the early stages of fracture healing (Lu et al., 2013). Most of the inspire

• naloxone hydrochloride We have demonstrated also in

  2018-11-08

  

  We have demonstrated also in this study that inhibition of the cellular antioxidant mechanism could promote differentiation of GICs. We also observed that GICs have an enhanced antioxidant mechanism compared to their serum-differentiated counterparts. Based on these findings, we speculate that GICs

• br Conclusions and future perspectives It

  2018-11-08

  

  Conclusions and future perspectives It has been suggested that developmental mechanisms are prisoners of their own phylogenetic histories (Goss, 1991). On the basis of recent studies of cardiac regeneration in fetal/neonatal mammals, it would appear that regenerative mechanisms are also prisoners

• We next asked what the impact of culture induced

  2018-11-08

  

  We next asked what the impact of culture-induced senescence of the fibroblasts was on the reprogramming efficiency. Four representative fibroblast lines from young (ages and 1year) and old (ages 81 and 83year) donors were propagated for up to 25 passages (Fig. 2A). The pluripotent reprogramming effi

• To date there have not been a consistent set of

  2018-11-08

  

  To date, there have not been a consistent set of EMF stimulus parameters used among research groups reported in the literature; however, results suggest that EMF promotion of bone ECM deposition in vitro is more far more efficient in osteoblasts differentiated from hBMSCs than from EZ Cap Reagent GG

• In addition to PES pre treatment increasing HPC adhesion

  2018-11-08

  

  In addition to PES pre-treatment increasing HPC7 adhesion within the colon, it also concomitantly reduced pulmonary presence, thereby reducing non-specific retention in healthy tissues. Pulmonary entrapment is a major obstacle for systemic HSPC delivery for regenerative purposes both experimentally

• br Resource table br Resource Details br Materials and http

  2018-11-08

  

  Resource table Resource Details Materials and methods Verification and authentication Resource table Resource Details Materials and methods Pluripotency assessment Sterility testing Verification and authentication Resource table Resource details Human skin fibrobl

• Overall ten genes five of which are coding genes

  2018-11-08

  

  Overall, ten genes, five of which are coding genes (FMOD, SULF1, COL12A1, STMN2 and MAEL), and five long-non-coding RNA (lncRNA) genes, were differentially expressed in hUC cells. lncRNAs are non-protein coding transcripts longer than 200 nucleotides. They have been implicated in the regulation of g

• In contrast to bona fide ESC lines the pre

  2018-11-08

  

  In order Asiatic acid to bona fide ESC lines, the pre-ESC lines expressed H19 and IGF2 mRNAs and lower levels of DNMT3B. This may reflect their limited ability to differentiate into the three germ layers, as a high H19 expression level has been associated with altered differentiation potential in p

• blasticidin A microsatellite PCR profile has

  2018-11-08

  

  A microsatellite PCR profile has been obtained for the cell line, and HLA Class I and II typing is available (Table 1). Blood group genotyping gave the blood group O1O1, expected to give rise to blood group O+ (Table 1). The cell line is free from mycoplasma contamination as determined by RT-qPCR.

• The following are the supplementary data related to this art

  2018-11-08

  

  The following are the supplementary data related to this article. Acknowledgments The authors wish to thank Dr. K. Inoue for the S100β/GFP-TG rats, Dr. A.F. Parlow and the NIDDK for the mdm2 inhibitor against pituitary hormones, Dr. S. Tanaka at Shizuoka University for the antibody against huma

• To test whether off target effect occurred in Abcc A

  2018-11-08

  

  To test whether off-target effect occurred in Abcc8-A4, a total of 8 potential off-target sites (POTs) for each sgRNA were predicted by the CRISPR design online tool. All POTs were PCR amplified and then sequenced. Sequencing results showed that no mutation was detected in these POTS (Fig. S1.A, B),

16072 records 1052/1072 page Previous Next First page 上5页 10511052105310541055 下5页 Last page